Epigenetic therapy of myelodysplastic syndromes connects to cellular differentiation independently of endogenous retroelement derepression.

myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) characterized by abnormal epigenetic repression and differentiation of bone marrow haematopoietic stem cells (HSCs). Drugs that reverse the epigenetic repression, such as 5-azacytidine (5-AZA), induces hematologic improvement in half of the patients treated.

Although the underlying mechanisms of therapeutic efficacy is not yet clear, the induction of endogenous retroelements (eres) has hypothesised.Using RNA sequencing (RNA-seq), we compared the transcriptional Eres in bone marrow HSCs of a new cohort of MDS and chronic myelomonocytic leukemia (CMML) patients before and after 5-AZA treatment with HSCs from healthy donors and patients with AML.

We further examined the ERE transcription using a comprehensive explanation ERE partially-overlapping transcripts expressed in HSCs, produced here by de novo assembly and supported by transcripts of full-length RNA-seq.Consistent with previous reports, we found that treatment with 5- AZA increased representation of ERE-derived RNA-seq reads in the transcriptome. However, the increase is comparable between treatment response and failure.

Extended view HSC transcription diversity offered by de novo assembly transcripts against Eres 5-AZA-responsive as a determinant of treatment outcome. In contrast, pre-treatment revealed expression and alternative splicing gene transcripts regulated as a predictor of response to the development of MDS and CMML patients to 5-AZA treatment.Our studies identify transcriptional signature-coding genes regulated development of non-protein-coding and not eres, as correlated good response from MDS and CMML patients for the treatment of 5-AZA and offer new candidates for further evaluation.

Dialysis membrane, 77mm

TX0113 2m, 2m
EUR 93.41

Dialysis Tubing No. 4 (DM003)

LA393-1MT 1 unit
EUR 7.6
Description: Dialysis Tubing No. 4 (DM003)

Dialysis Tubing Wash Buffer 5X

40120355-1 250 ml
EUR 27.08

Dialysis Tubing Wash Buffer 5X

40120355-2 500 ml
EUR 40.91

Dialysis Membrane-50 Av.flat width -

LA387-10MT 1 unit
EUR 60.05
Description: Dialysis Membrane-50 Av.flat width -

Dialysis Membrane-50 Av.flat width -

LA387-30MT 1 unit
EUR 161.71
Description: Dialysis Membrane-50 Av.flat width -

Dialysis Membrane-50 Av.flat width -

LA387-5MT 1 unit
EUR 34.79
Description: Dialysis Membrane-50 Av.flat width -

Dialysis Membrane-60 Av.flat width -

LA390-10MT 1 unit
EUR 60.11
Description: Dialysis Membrane-60 Av.flat width -

Dialysis Membrane-60 Av.flat width -

LA390-30MT 1 unit
EUR 166.51
Description: Dialysis Membrane-60 Av.flat width -

Dialysis Membrane-60 Av.flat width -

LA390-5MT 1 unit
EUR 34.79
Description: Dialysis Membrane-60 Av.flat width -

Dialysis Membrane-70 Av.flat width -

LA393-10MT 1 unit
EUR 60.11
Description: Dialysis Membrane-70 Av.flat width -

Dialysis Membrane-70 Av.flat width -

LA393-30MT 1 unit
EUR 166.51
Description: Dialysis Membrane-70 Av.flat width -

Dialysis Membrane-70 Av.flat width -

LA393-5MT 1 unit
EUR 34.79
Description: Dialysis Membrane-70 Av.flat width -

Dialysis Closure Clips a perfect

LA404-10NO 1 unit
EUR 125.96
Description: Dialysis Closure Clips a perfect

Dialysis Membrane-110 Av.flat width -

LA395-10MT 1 unit
EUR 66.93
Description: Dialysis Membrane-110 Av.flat width -

Dialysis Membrane-110 Av.flat width -

LA395-30MT 1 unit
EUR 188
Description: Dialysis Membrane-110 Av.flat width -

Dialysis Membrane-110 Av.flat width -

LA395-5MT 1 unit
EUR 39.89
Description: Dialysis Membrane-110 Av.flat width -

Dialysis Membrane-135 Av.flat width -

LA398-10MT 1 unit
EUR 65.57
Description: Dialysis Membrane-135 Av.flat width -

Dialysis Membrane-135 Av.flat width -

LA398-30MT 1 unit
EUR 184.29
Description: Dialysis Membrane-135 Av.flat width -

Dialysis Membrane-135 Av.flat width -

LA398-5MT 1 unit
EUR 47.67
Description: Dialysis Membrane-135 Av.flat width -

Dialysis Membrane-150 Av.flat width -

LA401-10MT 1 unit
EUR 83.76
Description: Dialysis Membrane-150 Av.flat width -

Dialysis Membrane-150 Av.flat width -

LA401-30MT 1 unit
EUR 235.13
Description: Dialysis Membrane-150 Av.flat width -

Dialysis Membrane-150 Av.flat width -

LA401-5MT 1 unit
EUR 47.67
Description: Dialysis Membrane-150 Av.flat width -

Dialysis Bagholder clip Beakers - PK6

BAF182370000-6EA PK6
EUR 42.53

Dialysis Membrane-135Av.flat width - 39.

LA398-1MT 1 unit
EUR 10.12
Description: Dialysis Membrane-135Av.flat width - 39.

Dialysis Membrane-150Av.flat width - 42.

LA401-1MT 1 unit
EUR 10.77
Description: Dialysis Membrane-150Av.flat width - 42.

GeBAflex Midi Dialysis, No Buffers (3500)

43270012-1 10 Tube(s)
EUR 87.58

GeBAflex Midi Dialysis, No Buffers (3500)

43270012-2 30 Tube(s)
EUR 180.66

GeBAflex Mini Dialysis, No Buffers (12000)

43270016-1 10 Tube(s)
EUR 43

GeBAflex Mini Dialysis, No Buffers (12000)

43270016-2 30 Tube(s)
EUR 85.98

Dialysis Membrane-50Av. flat width - 24.

LA387-1MT 1 unit
EUR 7.6
Description: Dialysis Membrane-50Av. flat width - 24.

Dialysis Membrane-60Av. flat width - 25.

LA390-1MT 1 unit
EUR 7.6
Description: Dialysis Membrane-60Av. flat width - 25.

Dialysis Membrane-110Av. flat width - 32

LA395-1MT 1 unit
EUR 8.73
Description: Dialysis Membrane-110Av. flat width - 32

Dialysis Tubing Size 1 Dia 8/32, 6.3 mm

LA653-10MT 1 unit
EUR 372.38
Description: Dialysis Tubing Size 1 Dia 8/32, 6.3 mm

Dialysis Tubing Size 1 Dia 8/32, 6.3 mm

LA653-30MT 1 unit
EUR 956.16
Description: Dialysis Tubing Size 1 Dia 8/32, 6.3 mm

Mini Dialysis Kit 1 kDa cutoff 2mL - EACH

80648394 EACH
EUR 625.05

Mini Dialysis Kit 8 kDa cutoff 2mL - EACH

80648432 EACH
EUR 625.05

Mini Dialysis Kit 1 kDa cutoff 250 ul - EACH

80648375 EACH
EUR 625.05

Mini Dialysis Kit 8 kDa cutoff 250 ul - EACH

80648413 EACH
EUR 625.05

GeBAflex Mini Dialysis, No Buffers (6000 MW cut off)

43270017-1 10 Tube (s) Ask for price

GeBAflex Mini Dialysis, No Buffers (6000 MW cut off)

43270017-2 30 Tube (s) Ask for price

Spectra/Por ® 2 Dry Standard RC Dialysis Tubing

LC9002-001 Roll
EUR 644.4

Spectra/Por ® 2 Dry Standard RC Dialysis Tubing

LC9002-002 Roll
EUR 644.4

Spectra/Por ® 2 Dry Standard RC Dialysis Tubing

LC9002-003 Roll
EUR 644.4

Spectra/Por ® 2 Dry Standard RC Dialysis Tubing

LC9002-004 Roll
EUR 1183.2

Spectra/Por ® 2 Dry Standard RC Dialysis Tubing

LC9002-005 Roll
EUR 1183.2

Spectra/Por ® 3 Dry Standard RC Dialysis Tubing

LC9003-001 Roll
EUR 588

Spectra/Por ® 3 Dry Standard RC Dialysis Tubing

LC9003-002 Roll
EUR 588

Spectra/Por ® 3 Dry Standard RC Dialysis Tubing

LC9003-003 Roll
EUR 616.8

Spectra/Por ® 4 Dry Standard RC Dialysis Tubing

LC9004-001 Roll
EUR 724.8

Spectra/Por ® 4 Dry Standard RC Dialysis Tubing

LC9004-002 Roll
EUR 465.6

Spectra/Por ® 4 Dry Standard RC Dialysis Tubing

LC9004-003 Roll
EUR 489.6

Spectra/Por ® 4 Dry Standard RC Dialysis Tubing

LC9004-004 Roll
EUR 547.2

Spectra/Por ® 4 Dry Standard RC Dialysis Tubing

LC9004-005 Roll
EUR 565.2

Spectra/Por ® 5 Dry Standard RC Dialysis Tubing

LC9005-001 Roll
EUR 517.2

Spectra/Por ® 5 Dry Standard RC Dialysis Tubing

LC9005-002 Roll
EUR 685.2

Visking Dialysis Tubing Clips - Gripping Width 44mm - PK10

TUB2040 PK10
EUR 48.03

Spectra/Por ® 1 Dry Standard RC Dialysis Tubing,15 m

LC9001-001 Roll
EUR 505.2

Spectra/Por ® 1 Dry Standard RC Dialysis Tubing, 30 m

LC9001-002 Roll
EUR 523.2

Spectra/Por ® 1 Dry Standard RC Dialysis Tubing, 30 m

LC9001-003 Roll
EUR 523.2

Spectra/Por ® 1 Dry Standard RC Dialysis Tubing, 30 m

LC9001-004 Roll
EUR 555.6

Spectra/Por ® 1 Dry Standard RC Dialysis Tubing, 30 m

LC9001-005 Roll
EUR 625.2

Spectra/Por ® 1 Dry Standard RC Dialysis Tubing, 15 m

LC9001-006 Roll
EUR 721.2

Spectra/Por ® 1 Dry Standard RC Dialysis Tubing, 15 m

LC9001-007 Roll
EUR 745.2

Visking Dialysis Tubing - 0.12 x 139.7mm (Wall Thickness x Diam) - 15m - EACH

TUB2038 EACH
EUR 431.83

Visking Dialysis Tubing - 0.03 x 14.3mm (Wall Thickness x Diam) - 30m - 30MTR

TUB2002 30MTR
EUR 102.82

Visking Dialysis Tubing - 0.03 x 15.9mm (Wall Thickness x Diam) - 30m - 30MTR

TUB2004 30MTR
EUR 130.72

Visking Dialysis Tubing - 0.03 x 17.5mm (Wall Thickness x Diam) - 30m - 30MTR

TUB2006 30MTR
EUR 118.97

Visking Dialysis Tubing - 0.03 x 19.0mm (Wall Thickness x Diam) - 30m - 30MTR

TUB2008 30MTR
EUR 104.28

Visking Dialysis Tubing - 0.03 x 21.5mm (Wall Thickness x Diam) - 30m - 30MTR

TUB2010 30MTR
EUR 126.32

Visking Dialysis Tubing - 0.03 x 23.8mm (Wall Thickness x Diam) - 30m - 30MTR

TUB2012 30MTR
EUR 117.5

Visking Dialysis Tubing - 0.03 x 25.4mm (Wall Thickness x Diam) - 30m - 30MTR

TUB2014 30MTR
EUR 117.5

Visking Dialysis Tubing - 0.03 x 28.6mm (Wall Thickness x Diam) - 30m - 30MTR

TUB2016 30MTR
EUR 124.85

Visking Dialysis Tubing - 0.04 x 31.7mm (Wall Thickness x Diam) - 30m - 15MTR

TUB2018 15MTR
EUR 111.63

Visking Dialysis Tubing - 0.04 x 38.1mm (Wall Thickness x Diam) - 30m - 15MTR

TUB2020 15MTR
EUR 165.97

Visking Dialysis Tubing - 0.05 x 50.8mm (Wall Thickness x Diam) - 15m - 15MTR

TUB2024 15MTR
EUR 165.97

Visking Dialysis Tubing - 0.09 x 69.8mm (Wall Thickness x Diam) - 30m - 15MTR

TUB2030 15MTR
EUR 267.32

Visking Dialysis Tubing - 0.12 x 82.5mm (Wall Thickness x Diam) - 15m - 15MTR

TUB2032 15MTR
EUR 267.32

Visking Dialysis Tubing - 0.12 x 95.2mm (Wall Thickness x Diam) - 15m - 15MTR

TUB2034 15MTR
EUR 336.36

Visking Dialysis Tubing - 0.12 x 114.3mm (Wall Thickness x Diam) - 15m - 15MTR

TUB2036 15MTR
EUR 368.67

Add cassette

1292236 1unit
EUR 280.06

iGFBP Cassette

INV-152 4.0mm (strip in a card) 25cards/box
EUR 1.42

LentiTitre Cassette

ATG-LT-02-10 10 cassettes
EUR 600

LentiTitre Cassette

ATG-LT-02-20 20 cassettes
EUR 1000

LentiTitre Cassette

ATG-LT-02-5 5 cassettes
EUR 315

BZO Test Cassette

BZO-D112 4.0mm (strip in a card) 25cards/box
EUR 0.27

AMP Test Cassette

AMP-1062 4.0mm (strip in a card) 25cards/box
EUR 0.28

THC Test Cassette

THC-1092 4.0mm (strip in a card) 25cards/box
EUR 0.27

MET Test Cassette

MET-1072 4.0mm (strip in a card) 25cards/box
EUR 0.28

Cassette Lilac - PK1000

HIS0117 PK1000
EUR 572.4

h-FABP Test Cassette

HF-1331 4.0mm (strip in a card) 25cards/box
EUR 1.91

Tissue Cassette IV, Blue

21468-250 250units
EUR 184

Tissue Cassette IV, Pink

21471-250 250units
EUR 172

FOB Rapid Test Cassette

TFO-602H 1 Test
EUR 1

HCV Rapid Test Cassette

IHC-402 40 Tests
EUR 14

TSH Rapid Test Cassette

OTS-402H 1 Test
EUR 1.5

Tissue Cassette IV, Yellow

21469-500 500units
EUR 177

Troponin I Test Cassette

TroponinI-1221 4.0mm (strip in a card) 25cards/box
EUR 0.76

Malaria pf/pv Ab Cassette

INV-911 4.0mm (strip in a card) 25cards/box
EUR 0.47

Syphilis Rapid Test Cassette

ISY-402 40 Tests
EUR 6

Ferritin Rapid test cassette

OFE-402H 1 Test
EUR 1.6

HIV 1.2 Rapid Test Cassette

IHI-402 40 Tests
EUR 16

CryoEM AutoGrid Cassette Puck

M-CEM-AGC-P1 1 EACH
EUR 587
Description: CryoEM AutoGrid Cassette Puck

SSRL Cassette Transfer Handle

M-CP-111-003 1 UNIT
EUR 460
Description: SSRL Cassette Transfer Handle

Chlamydia Rapid Test Cassette

ICH-502 20 Tests
EUR 11

Metal Lid for M480 Cassette

M481 1 ea.
EUR 169.2

Sub Blot Mini Cassette - EACH

SB10C EACH
EUR 68.85

Sub Blot Maxi Cassette - EACH

SB20C EACH
EUR 114.75

Simport Embedding Cassette Tan

HIS0038 PK1500
EUR 205.2

Simport Embedding Cassette Tan

HIS0049 PK1500
EUR 261.36

Simport Embedding Cassette Tan

HIS0060 PK1500
EUR 261.36

Simport Embedding Cassette Tan

HIS0071 PK1500
EUR 358.56

Simport Embedding Cassette Tan

HIS0082 PK1500
EUR 348.84

Simport Embedding Cassette Tan

HIS0104 PK1000
EUR 228.96

Simport Embedding Cassette Tan

HIS0115 PK1000
EUR 457.92

Simport Embedding Cassette Tan

HIS0126 PK1000
EUR 457.92

Simport Embedding Cassette Tan

HIS0181 PK2000
EUR 466.56

positive electrode cassette (red)

EHS1500-POS ea
EUR 139.2

Cassette Loading Station Insert

M-CEM-AGC-PLSI1 1 EACH
EUR 464
Description: Cassette Loading Station Insert

Simport Embedding Cassette Grey

HIS0039 PK1500
EUR 205.2

Simport Embedding Cassette Pink

HIS0044 PK1500
EUR 261.36

Simport Embedding Cassette Grey

HIS0050 PK1500
EUR 261.36

Simport Embedding Cassette Aqua

HIS0053 PK1500
EUR 261.36

Simport Embedding Cassette Pink

HIS0055 PK1500
EUR 261.36

Simport Embedding Cassette Blue

HIS0058 PK1500
EUR 249.48

Simport Embedding Cassette Grey

HIS0061 PK1500
EUR 261.36

Simport Embedding Cassette Aqua

HIS0064 PK1500
EUR 261.36

Simport Embedding Cassette Grey

HIS0072 PK1500
EUR 358.56

Simport Embedding Cassette Aqua

HIS0075 PK1500
EUR 358.56

Simport Embedding Cassette Grey

HIS0083 PK1500
EUR 348.84

Simport Embedding Cassette Aqua

HIS0086 PK1500
EUR 348.84

Simport Embedding Cassette Pink

HIS0099 PK1000
EUR 228.96

Simport Embedding Cassette Blue

HIS0102 PK1000
EUR 228.96

Simport Embedding Cassette Grey

HIS0105 PK1000
EUR 228.96

Simport Embedding Cassette Aqua

HIS0108 PK1000
EUR 228.96

Simport Embedding Cassette Pink

HIS0110 PK1000
EUR 457.92

Simport Embedding Cassette Blue

HIS0113 PK1000
EUR 457.92

Simport Embedding Cassette Grey

HIS0116 PK1000
EUR 457.92

Simport Embedding Cassette Aqua

HIS0119 PK1000
EUR 457.92

Simport Embedding Cassette Pink

HIS0121 PK1000
EUR 457.92

Simport Embedding Cassette Blue

HIS0124 PK1000
EUR 457.92

Simport Embedding Cassette Grey

HIS0127 PK1000
EUR 457.92

Simport Embedding Cassette Aqua

HIS0130 PK1000
EUR 457.92

Simport Embedding Cassette Pink

HIS0176 PK2000
EUR 466.56