Recombinant Human Vascular Endothelial Growth Factor (rh-VEGF165)

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF), is a potent mediator of angiogenesis and vasculogenesis in the fetus and adults. VEGF is a glycosylated mitogen that acts specifically on endothelial cells and has a variety of effects including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, and endothelial cell growth, promoting cell migration, and inhibiting apoptosis.

Six forms of human VEGF mRNA encoding VEGF proteins of 121, 145, 165, 183, 189, and 206 amino acids are produced from a single gene as a result of alternative splicing. VEGF165 appears to be the major genetic product found in human tissue and is the most potent angiogenic factor in the VEGF family.

EC50: Typically 2 ~ 5 ng / ml (Determined by human umbilical vein endothelial cells-HUVECs)

Biological activity: Fully biologically active compared to the standard. The ED50 as determined by a cell proliferation assay using human umbilical vein endothelial cells (HUVEC) is between 1.0-8.0 ng/ml.

Packaging: In general, recombinant proteins are supplied as a lyophilized powder which is shipped at room temperature.

Application: This product may be for research or further manufacturing use.

Stability and storage conditions:

Samples are stable for up to twelve months from date of receipt at -20 ° C to -80 ° C
Store it under sterile conditions at -20 ° C to -80 ° C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

1: rh-VEGF165 Concentration of RH-VEGF165 (ng / ml)

M: Protein marker standard Figure 2: Proliferation of UVEC examined by means of

Figure 1: Increase in the number of cells in the presence of rh-VEGF165 for 96h

Analysis of rh-VEGF165 by SDS-PAGE

Notes:

It is recommended that the product be reconstituted with sterile water to a final concentration of

0.5 mg / ml. Store the reconstituted product in aliquots at -20 ° C. Avoid multiple freeze-thaw cycles and exposure to frequent temperature changes.

The use of strong acids and alkalis, strong oxidants, and high concentrations of organic solvents should be avoided to protect the product from denaturation.

For research use only!

Frontier Advances:

A highly sensitive colorimetric aptasensor for the detection of vascular endothelial growth factor in human serum

Author: Dong Jiajia J, He Leiliang L, Wang Yilin Y, Yu Fei F, Yu Songcheng S, Liu Lie L, Wang Jia J, Tian Yongmei Y, Qu Lingbo L

Journal: Spectrochim Acta A Mol Biomol Spectrosc 2019-10-07 117622 226 DOI: 10.1002 / smll.201802403

Summary: Early detection of cancer is of great importance for the prevention and diagnosis of disease.

Orthogonal assay design for optimization of superparamagnetic chitosan plasmid gelatin microspheres that promote vascularization of artificial bones

Author: Tao Chen C, Lina Xie X, Changxuan Wang W, Cong Luo L, Xiaolan Yang Y, Tao Huang H

Journal: J. Biomed. Mater. Res. Part B Appl. Biomater. 2019-10-12 DOI: 10.1002 / small.201802403

Summary: + The optimal conditions for the preparation of the superparamagnetic chitosan plasmid (receiver-M29-VEGF165 / DH5a) have been determined.

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