Retroelement Insertion in a CRISPR/Cas9 Editing Site in the Early Embryo Intensifies Genetic Mosaicism.

Continued CRISPR/Cas9-mediated enhancing exercise that enables differential and asynchronous modification of alleles in successive cell generations expands allelic complexity. To perceive the earliest occasions throughout CRISPR/Cas9 enhancing and the allelic choice amongst the progeny of subsequent cell divisions, we inspected in element the genotypes of 4- and 8-cell embryos and embryonic stem cells (ESCs) after microinjection of a CRISPR toolkit into the zygotes.

We discovered a increased enhancing frequency in 8-cell embryos than in 4-cell embryos, indicating that the CRISPR/Cas9 exercise endured by the 8-cell stage. Analysis of a CRISPR/Cas9 transgenic founder mouse revealed that 4 totally different alleles had been current in its organs in totally different mixtures and that its germline included three totally different mutant alleles, as proven by the genotypes of the pups.

The indel depth, which measured the extent of indels at the sequence degree inside single embryos, decreased considerably as the embryos superior to type ESCs, suggesting that exclusion of deadly indels occurred in the subsequent cell generations. Interestingly, we found that the CRISPR websites continuously contained launched retroelement sequences and that this occurred preferentially with sure lessons of retroelements.

Therefore, in addition to CRISPR/Cas9’s innate mechanism of separate, differential enzymatic modifications of alleles, the frequent retroelement insertions that happen in early mouse embryos throughout CRISPR/Cas9 enhancing additional broaden the allelic variety and mosaicism in the ensuing transgenic founders.

Retroelement Insertion in a CRISPR/Cas9 Editing Site in the Early Embryo Intensifies Genetic Mosaicism.
Retroelement Insertion in a CRISPR/Cas9 Editing Site in the Early Embryo Intensifies Genetic Mosaicism.

DiD (or DiIC18(5)): (50mg)

60014 50MG
EUR 161
Description: Minimum order quantity: 1 unit of 50MG

DID ELISA Kit (Rat) : 96 Wells (OKEH02470)

OKEH02470 96 Wells
EUR 779
Description: Description of target: This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. A similar protein in human, mouse, and pig has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, the protein can function as a protease.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Competitive ELISA;Sensitivity: 0.47 ng/mL

DID ELISA Kit (Mouse) : 96 Wells (OKEH00588)

OKEH00588 96 Wells
EUR 779
Description: Description of target: ;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.46 ng/mL

DiD labeling solution [1,1-Dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine] *5 mM DMSO solution*

22033 10 mL
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Recombinant HPV HPV 11 Protein, GST, E.coli-100ug

QP12306-100ug 100ug
EUR 218

Recombinant HPV HPV 11 Protein, GST, E.coli-1mg

QP12306-1mg 1mg
EUR 1261

Recombinant HPV HPV 11 Protein, GST, E.coli-500ug

QP12306-500ug 500ug
EUR 663

Recombinant HPV HPV 16 Protein, GST, E.coli-100ug

QP12307-100ug 100ug
EUR 218

Recombinant HPV HPV 16 Protein, GST, E.coli-1mg

QP12307-1mg 1mg
EUR 1261

Recombinant HPV HPV 16 Protein, GST, E.coli-500ug

QP12307-500ug 500ug
EUR 663

Recombinant HPV HPV 18 Protein, GST, E.coli-100ug

QP12308-100ug 100ug
EUR 218

Recombinant HPV HPV 18 Protein, GST, E.coli-1mg

QP12308-1mg 1mg
EUR 1261

Recombinant HPV HPV 18 Protein, GST, E.coli-500ug

QP12308-500ug 500ug
EUR 663

Recombinant HPV HPV 6 Protein, GST, E.coli-100ug

QP12309-100ug 100ug
EUR 218

Recombinant HPV HPV 6 Protein, GST, E.coli-1mg

QP12309-1mg 1mg
EUR 1261

Recombinant HPV HPV 6 Protein, GST, E.coli-500ug

QP12309-500ug 500ug
EUR 663

HPV-E6-C

5-01338 4 x 1mg Ask for price

HPV-E6-M

5-01339 4 x 1mg Ask for price

HPV-E6-N

5-01340 4 x 1mg Ask for price

HPV-E7-C

5-01341 4 x 1mg Ask for price

HPV-E7-N

5-01342 4 x 1mg Ask for price

HPV type 1

DAG205 1 ml
EUR 937

HPV type 2

DAG206 1 ml
EUR 881

HPV type 3

DAG207 1 ml
EUR 881

HPV type 2

DAG4703 0.25 mg
EUR 720

HPV type 3

DAG4704 0.25 mg
EUR 720

HPV-16 Antibody

abx021894-1mg 1 mg
EUR 843
  • Shipped within 5-10 working days.

HPV-16 Antibody

abx021895-1mg 1 mg
EUR 801
  • Shipped within 5-10 working days.

HPV-E7-N

SP-100307-1 1 mg
EUR 225

HPV-E7-C

SP-100308-1 1 mg
EUR 225

HPV-E6-N

SP-100309-1 1 mg
EUR 286

HPV-E6-M

SP-100310-1 1 mg
EUR 225

HPV-E6-C

SP-100311-1 1 mg
EUR 164

Anti-HPV antibody

STJ180126 0.1 ml
EUR 266

Anti-HPV antibody

STJ180404 0.1 ml
EUR 229

HPV type 16 [GST]

DAG1573 100 µg
EUR 645

HPV type 18 [GST]

DAG1574 100 µg
EUR 645

HPV type 11 [GST]

DAG1576 100 µg
EUR 645

HPV-16 E2 Antibody

abx021892-1mg 1 mg
EUR 599
  • Shipped within 5-10 working days.

HPV-16 E3 Antibody

abx021893-1mg 1 mg
EUR 704
  • Shipped within 5-10 working days.

HPV-16 E7 Antibody

abx021896-1mg 1 mg
EUR 704
  • Shipped within 5-10 working days.

HPV-16 L1 Antibody

abx021897-1mg 1 mg
EUR 599
  • Shipped within 5-10 working days.

HPV-16 L1 Antibody

abx021898-1mg 1 mg
EUR 599
  • Shipped within 5-10 working days.

HPV-18 E6 Antibody

abx021899-1mg 1 mg
EUR 599
  • Shipped within 5-10 working days.

HPV-18 E6 Antibody

abx021900-1mg 1 mg
EUR 599
  • Shipped within 5-10 working days.

HPV-18 E7 Antibody

abx021901-1mg 1 mg
EUR 878
  • Shipped within 5-10 working days.

HPV 18 L1 Protein

abx060602-100ug 100 ug
EUR 1970
  • Shipped within 5-10 working days.

Inactivated HPV-2 Antigen

VAng-Lsx0262-inquire inquire Ask for price
Description: HPV type 2, native virus from Vero cells.

Recombinant HPV-2 Protein

VAng-Lsx0263-inquire inquire Ask for price
Description: HPV type 2, recombinant protein from MA 104 cells.

Inactivated HPV-3 Antigen

VAng-Lsx0264-inquire inquire Ask for price
Description: HPV type 3, native virus from Vero cells.

Recombinant HPV-3 Protein

VAng-Lsx0265-inquire inquire Ask for price
Description: HPV type 3, recombinant protein from MA 104 cells.

VAC-ID Gardasil Human Papilloma Virus Vaccine (Quadrivalent 6+11+16+18) identification ELISA kit, 24 tests

550-HPV-VID-24 1 Kit Ask for price

Human papillomavirus,HPV ELISA Kit

201-12-1101 96 tests
EUR 440
  • This papillomavirus ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

HPV-11 E7 Protein Antibody

abx021927-1mg 1 mg
EUR 739
  • Shipped within 5-10 working days.

HPV-11 E7 Protein Antibody

abx021928-1mg 1 mg
EUR 739
  • Shipped within 5-10 working days.

HPV-16 E7 Protein Antibody

abx021929-1mg 1 mg
EUR 739
  • Shipped within 5-10 working days.

HPV-16 E7 Protein Antibody

abx021930-1mg 1 mg
EUR 732
  • Shipped within 5-10 working days.

HPV-16 E7 Protein Antibody

abx021931-1mg 1 mg
EUR 739
  • Shipped within 5-10 working days.

HPV-18 E7 Protein Antibody

abx021933-1mg 1 mg
EUR 739
  • Shipped within 5-10 working days.

HPV-18 E7 Protein Antibody

abx021934-1mg 1 mg
EUR 739
  • Shipped within 5-10 working days.

Human Papillomavirus (HPV) ELISA Kit

abx052328-96tests 96 tests
EUR 668
  • Shipped within 5-10 working days.

Human papillomavirus(HPV)ELISA Kit

GA-E1117HM-48T 48T
EUR 289

Human papillomavirus(HPV)ELISA Kit

GA-E1117HM-96T 96T
EUR 466

Recombinant HPV-66 E1 Protein

VAng-Wyb3368-inquire inquire Ask for price
Description: Human papillomavirus type 66 Replication protein E1, recombinant protein.

Recombinant HPV-66 L1 Protein

VAng-Wyb3372-inquire inquire Ask for price
Description: Human papillomavirus type 66 Major capsid protein L1, recombinant protein.

Recombinant HPV-67 L1 Protein

VAng-Wyb3374-inquire inquire Ask for price
Description: Human papillomavirus type 67 Major capsid protein L1, recombinant protein.

Recombinant HPV-68 L1 Protein

VAng-Wyb3378-inquire inquire Ask for price
Description: Human papillomavirus type 68 Major capsid protein L1, recombinant protein.

Recombinant HPV-69 E1 Protein

VAng-Wyb3379-inquire inquire Ask for price
Description: Human papillomavirus type 69 Replication protein E1, recombinant protein.

Recombinant HPV-69 L1 Protein

VAng-Wyb3383-inquire inquire Ask for price
Description: Human papillomavirus type 69 Major capsid protein L1, recombinant protein.

Recombinant HPV-6a E1 Protein

VAng-Wyb3385-inquire inquire Ask for price
Description: Human papillomavirus type 6a Replication protein E1, recombinant protein.

Recombinant HPV-6b E1 Protein

VAng-Wyb3393-inquire inquire Ask for price
Description: Human papillomavirus type 6b Replication protein E1, recombinant protein.

Recombinant HPV-70 E1 Protein

VAng-Wyb3410-inquire inquire Ask for price
Description: Human papillomavirus type 70 Replication protein E1, recombinant protein.

Recombinant HPV-70 L1 Protein

VAng-Wyb3415-inquire inquire Ask for price
Description: Human papillomavirus type 70 Major capsid protein L1, recombinant protein.

Recombinant HPV-72 E1 Protein

VAng-Wyb3417-inquire inquire Ask for price
Description: Human papillomavirus type 72 Replication protein E1, recombinant protein.

Recombinant HPV-73 E1 Protein

VAng-Wyb3418-inquire inquire Ask for price
Description: Human papillomavirus type 73 Replication protein E1, recombinant protein.

Recombinant HPV-8 E1 Protein

VAng-Wyb3419-inquire inquire Ask for price
Description: Human papillomavirus type 8 Replication protein E1, recombinant protein.

Recombinant HPV-8 L1 Protein

VAng-Wyb3424-inquire inquire Ask for price
Description: Human papillomavirus type 8 Major capsid protein L1, recombinant protein.

Recombinant HPV-8 L2 Protein

VAng-Wyb3425-inquire inquire Ask for price
Description: Human papillomavirus type 8 Minor capsid protein L2, recombinant protein.

Recombinant HPV-82 L1 Protein

VAng-Wyb3426-inquire inquire Ask for price
Description: Human papillomavirus type 82 Major capsid protein L1, recombinant protein.

Recombinant HPV-9 E1 Protein

VAng-Wyb3428-inquire inquire Ask for price
Description: Human papillomavirus type 9 Replication protein E1, recombinant protein.

Recombinant HPV-9 L1 Protein

VAng-Wyb3432-inquire inquire Ask for price
Description: Human papillomavirus type 9 Major capsid protein L1, recombinant protein.

Recombinant HPV-9 L2 Protein

VAng-Wyb3433-inquire inquire Ask for price
Description: Human papillomavirus type 9 Minor capsid protein L2, recombinant protein.

Recombinant HPV-ME180 L1 Protein

VAng-Wyb3438-inquire inquire Ask for price
Description: Human papillomavirus type ME180 Major capsid protein L1, recombinant protein.

Recombinant HPV-RTRX7 E1 Protein

VAng-Wyb3440-inquire inquire Ask for price
Description: Human papillomavirus type RTRX7 Replication protein E1, recombinant protein.

Recombinant HPV-10 E1 Protein

VAng-Wyb3000-inquire inquire Ask for price
Description: Human papillomavirus type 10 Replication protein E1, recombinant protein.

Recombinant HPV-10 L1 Protein

VAng-Wyb3004-inquire inquire Ask for price
Description: Human papillomavirus type 10 Major capsid protein L1, recombinant protein.

Recombinant HPV-11 E1 Protein

VAng-Wyb3006-inquire inquire Ask for price
Description: Human papillomavirus type 11 Replication protein E1, recombinant protein.

Recombinant HPV-12 L1 Protein

VAng-Wyb3019-inquire inquire Ask for price
Description: Human papillomavirus type 12 Major capsid protein L1, recombinant protein.

Recombinant HPV-12 L2 Protein

VAng-Wyb3020-inquire inquire Ask for price
Description: Human papillomavirus type 12 Minor capsid protein L2, recombinant protein.

Recombinant HPV-13 E1 Protein

VAng-Wyb3021-inquire inquire Ask for price
Description: Human papillomavirus type 13 Replication protein E1, recombinant protein.

Recombinant HPV-14 E1 Protein

VAng-Wyb3023-inquire inquire Ask for price
Description: Human papillomavirus type 14 Replication protein E1, recombinant protein.

Recombinant HPV-14 L1 Protein

VAng-Wyb3026-inquire inquire Ask for price
Description: Human papillomavirus type 14 Major capsid protein L1, recombinant protein.

Recombinant HPV-14 L2 Protein

VAng-Wyb3027-inquire inquire Ask for price
Description: Human papillomavirus type 14 Minor capsid protein L2, recombinant protein.

Recombinant HPV-15 E1 Protein

VAng-Wyb3028-inquire inquire Ask for price
Description: Human papillomavirus type 15 Replication protein E1, recombinant protein.

Recombinant HPV-15 L2 Protein

VAng-Wyb3032-inquire inquire Ask for price
Description: Human papillomavirus type 15 Minor capsid protein L2, recombinant protein.

Recombinant HPV-16 E1 Protein

VAng-Wyb3033-inquire inquire Ask for price
Description: Human papillomavirus type 16 Replication protein E1, recombinant protein.

Recombinant HPV-16 E6 Protein

VAng-Wyb3038-100g 100 µg
EUR 669
Description: Human papillomavirus 16 protein E6, recombinant protein.

Recombinant HPV-16 E6 Protein

VAng-Wyb3038-1mg 1 mg
EUR 3404
Description: Human papillomavirus 16 protein E6, recombinant protein.

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