Frontiers of Retrovirology

Complex retroviruses, retroelements, HIV p24 and their hosts

Retroelement Insertion in a CRISPR/Cas9 Editing Site in the Early Embryo Intensifies Genetic Mosaicism.
Retroelement Insertion in a CRISPR/Cas9 Editing Site in the Early Embryo Intensifies Genetic Mosaicism. 

Retroelement Insertion in a CRISPR/Cas9 Editing Site in the Early Embryo Intensifies Genetic Mosaicism.

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Continued CRISPR/Cas9-mediated enhancing exercise that enables differential and asynchronous modification of alleles in successive cell generations expands allelic complexity. To perceive the earliest occasions throughout CRISPR/Cas9 enhancing and the allelic choice amongst the progeny of subsequent cell divisions, we inspected in element the genotypes of 4- and 8-cell embryos and embryonic stem cells (ESCs) after microinjection of a CRISPR toolkit into the zygotes.

We discovered a increased enhancing frequency in 8-cell embryos than in 4-cell embryos, indicating that the CRISPR/Cas9 exercise endured by the 8-cell stage. Analysis of a CRISPR/Cas9 transgenic founder mouse revealed that 4 totally different alleles had been current in its organs in totally different mixtures and that its germline included three totally different mutant alleles, as proven by the genotypes of the pups.

The indel depth, which measured the extent of indels at the sequence degree inside single embryos, decreased considerably as the embryos superior to type ESCs, suggesting that exclusion of deadly indels occurred in the subsequent cell generations. Interestingly, we found that the CRISPR websites continuously contained launched retroelement sequences and that this occurred preferentially with sure lessons of retroelements.

Therefore, in addition to CRISPR/Cas9’s innate mechanism of separate, differential enzymatic modifications of alleles, the frequent retroelement insertions that happen in early mouse embryos throughout CRISPR/Cas9 enhancing additional broaden the allelic variety and mosaicism in the ensuing transgenic founders.

Retroelement Insertion in a CRISPR/Cas9 Editing Site in the Early Embryo Intensifies Genetic Mosaicism.
Retroelement Insertion in a CRISPR/Cas9 Editing Site in the Early Embryo Intensifies Genetic Mosaicism.

DiD (or DiIC18(5)): (50mg)
60014 50MG
EUR 161.00
Description: Minimum order quantity: 1 unit of 50MG

DID ELISA Kit (Mouse) : 96 Wells (OKEH00588)
OKEH00588 96 Wells
EUR 779.00
Description: Description of target: ;Species reactivity: Mouse;Application: ;Assay info: Assay Methodology: Quantitative Sandwich ELISA;Sensitivity: 0.46 ng/mL

DID ELISA Kit (Rat) : 96 Wells (OKEH02470)
OKEH02470 96 Wells
EUR 779.00
Description: Description of target: This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. A similar protein in human, mouse, and pig has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, the protein can function as a protease.;Species reactivity: Rat;Application: ;Assay info: Assay Methodology: Quantitative Competitive ELISA;Sensitivity: 0.47 ng/mL

DiD labeling solution [1,1-Dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine] *5 mM DMSO solution*
22033 10 mL
EUR 219.00
  • R-phrase: R20, R21, R22
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  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

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Recombinant HPV-8 L1 Protein
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Recombinant HPV-82 L1 Protein
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Recombinant HPV-9 E1 Protein
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Description: Human papillomavirus type 9 Major capsid protein L1, recombinant protein.

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Recombinant HPV-ME180 L1 Protein
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Recombinant HPV-RTRX7 E1 Protein
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Recombinant HPV-10 E1 Protein
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Description: Human papillomavirus type 10 Major capsid protein L1, recombinant protein.

Recombinant HPV-11 E1 Protein
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Recombinant HPV-12 L1 Protein
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Description: Human papillomavirus type 12 Major capsid protein L1, recombinant protein.

Recombinant HPV-12 L2 Protein
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Recombinant HPV-13 E1 Protein
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Recombinant HPV-14 E1 Protein
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Recombinant HPV-14 L1 Protein
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Description: Human papillomavirus type 14 Major capsid protein L1, recombinant protein.

Recombinant HPV-14 L2 Protein
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Description: Human papillomavirus type 14 Minor capsid protein L2, recombinant protein.

Recombinant HPV-15 E1 Protein
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Description: Human papillomavirus type 15 Replication protein E1, recombinant protein.

Recombinant HPV-15 L2 Protein
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Description: Human papillomavirus type 15 Minor capsid protein L2, recombinant protein.

Recombinant HPV-16 E1 Protein
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Description: Human papillomavirus type 16 Replication protein E1, recombinant protein.

Recombinant HPV-16 E6 Protein
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EUR 669.00
Description: Human papillomavirus 16 protein E6, recombinant protein.

Recombinant HPV-16 E6 Protein
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EUR 3404.00
Description: Human papillomavirus 16 protein E6, recombinant protein.

Recombinant HPV-16 E6 Protein
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EUR 1824.00
Description: Human papillomavirus 16 protein E6, recombinant protein.

Recombinant HPV-16 L1 Protein
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Description: Human papillomavirus type 16 Major capsid protein L1, recombinant protein.

Recombinant HPV-17 L1 Protein
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Description: Human papillomavirus type 17 Major capsid protein L1, recombinant protein.

Recombinant HPV-17 L2 Protein
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Description: Human papillomavirus type 17 Minor capsid protein L2, recombinant protein.

Recombinant HPV-18 E1 Protein
VAng-Wyb3055-200gEcoli 200 µg (E. coli)
EUR 3885.00
Description: Human papillomavirus type 18 Replication protein E1, recombinant protein.

Recombinant HPV-18 E1 Protein
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EUR 2649.00
Description: Human papillomavirus type 18 Replication protein E1, recombinant protein.

Recombinant HPV-19 L2 Protein
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Description: Human papillomavirus type 19 Minor capsid protein L2, recombinant protein.

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Description: Human papillomavirus type 1a Replication protein E1, recombinant protein.

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Description: Human papillomavirus type 1a Major capsid protein L1, recombinant protein.

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Description: Human papillomavirus type 1a Minor capsid protein L2, recombinant protein.

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Description: Human papillomavirus type 20 Major capsid protein L1, recombinant protein.

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Description: Human papillomavirus type 20 Minor capsid protein L2, recombinant protein.

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Description: Human papillomavirus type 21 Regulatory protein E2, recombinant protein.

Recombinant HPV-21 L1 Protein
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Description: Human papillomavirus type 21 Major capsid protein L1, recombinant protein.

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Description: Human papillomavirus type 21 Minor capsid protein L2, recombinant protein.

Recombinant HPV-22 E1 Protein
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Description: Human papillomavirus type 22 Major capsid protein L1, recombinant protein.

Recombinant HPV-22 L2 Protein
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Description: Human papillomavirus type 22 Minor capsid protein L2, recombinant protein.

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Description: Human papillomavirus type 23 Replication protein E1, recombinant protein.

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Description: Human papillomavirus type 23 Major capsid protein L1, recombinant protein.

Recombinant HPV-23 L2 Protein
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Description: Human papillomavirus type 23 Minor capsid protein L2, recombinant protein.

Recombinant HPV-24 E1 Protein
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Recombinant HPV-24 L1 Protein
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Description: Human papillomavirus type 24 Major capsid protein L1, recombinant protein.

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Description: Human papillomavirus type 24 Minor capsid protein L2, recombinant protein.

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Description: Human papillomavirus type 25 Replication protein E1, recombinant protein.

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Description: Human papillomavirus type 25 Regulatory protein E2, recombinant protein.

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Description: Human papillomavirus type 25 Minor capsid protein L2, recombinant protein.

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Description: Human papillomavirus type 26 Replication protein E1, recombinant protein.

Recombinant HPV-26 L1 Protein
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Description: Human papillomavirus type 26 Major capsid protein L1, recombinant protein.

Recombinant HPV-27 E1 Protein
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Description: Human papillomavirus type 27 Replication protein E1, recombinant protein.

Recombinant HPV-27 L1 Protein
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Description: Human papillomavirus type 27 Major capsid protein L1, recombinant protein.

Recombinant HPV-28 E1 Protein
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Description: Human papillomavirus type 28 Replication protein E1, recombinant protein.

Recombinant HPV-28 L1 Protein
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Description: Human papillomavirus type 28 Major capsid protein L1, recombinant protein.

Recombinant HPV-29 E1 Protein
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Description: Human papillomavirus type 29 Replication protein E1, recombinant protein.

Recombinant HPV-29 L1 Protein
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Description: Human papillomavirus type 29 Major capsid protein L1, recombinant protein.

Recombinant HPV-2a E1 Protein
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Description: Human papillomavirus type 2a Replication protein E1, recombinant protein.

Recombinant HPV-2a L1 Protein
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Description: Human papillomavirus type 2a Major capsid protein L1, recombinant protein.

Recombinant HPV-3 E1 Protein
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Description: Human papillomavirus type 3 Replication protein E1, recombinant protein.

Recombinant HPV-3 L1 Protein
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Description: Human papillomavirus type 3 Major capsid protein L1, recombinant protein.

Recombinant HPV-30 L1 Protein
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Description: Human papillomavirus type 30 Major capsid protein L1, recombinant protein.

Recombinant HPV-31 E1 Protein
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Description: Human papillomavirus type 31 Replication protein E1, recombinant protein.

Recombinant HPV-31 L1 Protein
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Description: Human papillomavirus type 31 Major capsid protein L1, recombinant protein.

Recombinant HPV-32 E1 Protein
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Description: Human papillomavirus type 32 Replication protein E1, recombinant protein.

Recombinant HPV-32 L1 Protein
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Description: Human papillomavirus type 32 Major capsid protein L1, recombinant protein.

Recombinant HPV-33 E1 Protein
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Description: Human papillomavirus type 33 Replication protein E1, recombinant protein.

Recombinant HPV-34 E1 Protein
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Description: Human papillomavirus type 34 Replication protein E1, recombinant protein.

Recombinant HPV-34 L1 Protein
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Description: Human papillomavirus type 34 Major capsid protein L1, recombinant protein.

Recombinant HPV-35 E1 Protein
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Description: Human papillomavirus type 35 Replication protein E1, recombinant protein.

Recombinant HPV-35 L1 Protein
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Description: Human papillomavirus type 35 Major capsid protein L1, recombinant protein.

Recombinant HPV-36 E1 Protein
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Description: Human papillomavirus type 36 Replication protein E1, recombinant protein.

Recombinant HPV-36 E2 Protein
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Description: Human papillomavirus type 36 Regulatory protein E2, recombinant protein.

Recombinant HPV-36 L1 Protein
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Description: Human papillomavirus type 36 Major capsid protein L1, recombinant protein.

Recombinant HPV-36 L2 Protein
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Description: Human papillomavirus type 36 Minor capsid protein L2, recombinant protein.

Recombinant HPV-37 E1 Protein
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Description: Human papillomavirus type 37 Replication protein E1, recombinant protein.

Recombinant HPV-37 L1 Protein
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Description: Human papillomavirus type 37 Major capsid protein L1, recombinant protein.

Recombinant HPV-37 L2 Protein
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Description: Human papillomavirus type 37 Minor capsid protein L2, recombinant protein.

Recombinant HPV-38 E1 Protein
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Description: Human papillomavirus type 38 Replication protein E1, recombinant protein.

Recombinant HPV-38 L1 Protein
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Description: Human papillomavirus type 38 Major capsid protein L1, recombinant protein.

Recombinant HPV-38 L2 Protein
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Description: Human papillomavirus type 38 Minor capsid protein L2, recombinant protein.

Recombinant HPV-39 E1 Protein
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Description: Human papillomavirus type 39 Replication protein E1, recombinant protein.

Recombinant HPV-39 L1 Protein
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Description: Human papillomavirus type 39 Major capsid protein L1, recombinant protein.

Recombinant HPV-40 E1 Protein
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Description: Human papillomavirus type 40 Replication protein E1, recombinant protein.

Recombinant HPV-40 L1 Protein
VAng-Wyb3227-inquire inquire Ask for price
Description: Human papillomavirus type 40 Major capsid protein L1, recombinant protein.

Recombinant HPV-41 E1 Protein
VAng-Wyb3229-inquire inquire Ask for price
Description: Human papillomavirus type 41 Replication protein E1, recombinant protein.

Recombinant HPV-41 L1 Protein
VAng-Wyb3235-inquire inquire Ask for price
Description: Human papillomavirus type 41 Major capsid protein L1, recombinant protein.

Recombinant HPV-41 L2 Protein
VAng-Wyb3236-inquire inquire Ask for price
Description: Human papillomavirus type 41 Minor capsid protein L2, recombinant protein.

Recombinant HPV-42 E1 Protein
VAng-Wyb3237-inquire inquire Ask for price
Description: Human papillomavirus type 42 Replication protein E1, recombinant protein.

Recombinant HPV-42 L1 Protein
VAng-Wyb3243-inquire inquire Ask for price
Description: Human papillomavirus type 42 Major capsid protein L1, recombinant protein.

Recombinant HPV-43 L1 Protein
VAng-Wyb3246-inquire inquire Ask for price
Description: Human papillomavirus type 43 Major capsid protein L1, recombinant protein.

Recombinant HPV-44 E1 Protein
VAng-Wyb3248-inquire inquire Ask for price
Description: Human papillomavirus type 44 Replication protein E1, recombinant protein.

Recombinant HPV-45 E1 Protein
VAng-Wyb3254-inquire inquire Ask for price
Description: Human papillomavirus type 45 Replication protein E1, recombinant protein.

Recombinant HPV-45 L1 Protein
VAng-Wyb3258-inquire inquire Ask for price
Description: Human papillomavirus type 45 Major capsid protein L1, recombinant protein.

Recombinant HPV-47 E1 Protein
VAng-Wyb3260-inquire inquire Ask for price
Description: Human papillomavirus type 47 Replication protein E1, recombinant protein.

Recombinant HPV-47 E2 Protein
VAng-Wyb3261-inquire inquire Ask for price
Description: Human papillomavirus type 47 Regulatory protein E2, recombinant protein.

Recombinant HPV-47 L1 Protein
VAng-Wyb3265-inquire inquire Ask for price
Description: Human papillomavirus type 47 Major capsid protein L1, recombinant protein.

Recombinant HPV-47 L2 Protein
VAng-Wyb3266-inquire inquire Ask for price
Description: Human papillomavirus type 47 Minor capsid protein L2, recombinant protein.

Recombinant HPV-48 E1 Protein
VAng-Wyb3267-inquire inquire Ask for price
Description: Human papillomavirus type 48 Replication protein E1, recombinant protein.

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