Structure of KAP1 tripartite motif identifies molecular interfaces required for retroelement silencing.

Transcription of transposable parts is tightly regulated to stop genome injury. KRAB domain-containing zinc finger proteins (KRAB-ZFPs) and KRAB-associated protein 1 (KAP1/TRIM28) play a key position in regulating retrotransposons. KRAB-ZFPs acknowledge particular retrotransposon sequences and recruit KAP1, inducing the meeting of an epigenetic silencing advanced, with chromatin transforming actions that repress transcription of the focused retrotransposon and adjoining genes.

Our biophysical and structural knowledge present that the tripartite motif (TRIM) of KAP1 kinds antiparallel dimers, which additional assemble into tetramers and higher-order oligomers in a concentration-dependent method. Structure-based mutations within the B-box 1 area forestall higher-order oligomerization with out important loss of retrotransposon silencing exercise, indicating that, in distinction to different TRIM-family proteins, self-assembly just isn’t important for KAP1 operate.

The crystal construction of the KAP1 TRIM dimer identifies the KRAB area binding website within the coiled-coil area close to the dyad. Mutations at this website abolished KRAB binding and transcriptional silencing exercise of KAP1.

Structure of KAP1 tripartite motif identifies molecular interfaces required for retroelement silencing.
Structure of KAP1 tripartite motif identifies molecular interfaces required for retroelement silencing.

This work identifies the interplay interfaces within the KAP1 TRIM accountable for self-association and KRAB binding and establishes their position in retrotransposon silencing.

Dichlorofluorescein I

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2',7'-Dichlorofluorescein

11716-71 1G
EUR 17.15

2'',7''-Dichlorofluorescein

GT8033-1 1
EUR 22.1

2',7'-Dichlorofluorescein

GT8033-1G 1 g
EUR 62.4

2 7-Dichlorofluorescein

D15310 5G
EUR 134.99

2, 7-Dichlorofluorescein

DD1220 5g
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USP Sol. Dichlorofluorescein TS - 100ML

USP2301 100ML
EUR 375.3

USP Sol. Dichlorofluorescein TS - 500ML

USP2305 500ML
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2’,7’-Dichlorofluorescein, Hi-AR™

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2',7'-Dichlorofluorescein diacetate

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5-(&6)-Carboxy-2', 7'-Dichlorofluorescein

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5-(&6)-Carboxy-2', 7'-Dichlorofluorescein

51016-1 EA
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5(6)-Carboxy-2',7'-dichlorofluorescein

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5-Carboxy-2'',7''-dichlorofluorescein DA

GW5240-100 100
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5-Carboxy-2'',7''-dichlorofluorescein DA

GW5240-250 250
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E37F235 100-mg
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CDCF [5-(and-6)-Carboxy-2',7'-dichlorofluorescein] *Mixed isomers*

235-100mg 100 mg
EUR 109
Description: CDCF [5(6)-carboxy-2',7'-dichlorofluorescein] is the fluorescent product of 5(6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate and 5(6)-carboxy-2',7'-dichlorofluorescein diacetate in cells.

5(6)-Carboxy-2',7'-Dichlorofluorescein Diacetate Succinimidyl Ester

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6-Carboxy-2’,7’-dichlorofluorescein 3’,6’-Diacetate Succinimidyl Ester

C177890 250mg
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CDCFDA [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate] *Mixed isomers*

22025-100mg 100 mg
EUR 109
Description: It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample.

CDCFDA-[5-(and-6)-Carboxy-2',7'-dichlorofluorescein-diacetate]-*Mixed-isomers*

E37F22025 100-mg
EUR 398.33
Description: Cell-staining.-Cytoplasmic-staining.

CDCFDA, SE [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester] *Mixed isomers*

22026-25mg 25 mg
EUR 109
Description: It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample.

CDCFDA,-SE-[5-(and-6)-Carboxy-2',7'-dichlorofluorescein-diacetate,-succinimidyl-ester]-*Mixed-isomers*

E37F22026 25-mg
EUR 533.33
Description: Cell-staining.-Cytoplasmic-staining.

5-(AND-6)-CARBOXY-2', 7'-DICHLOROFLUORESC

90040-1 EA
EUR 188

2',7'-Dichlorofluorescin diacetate

D15325 50MG
EUR 203.85

2'',7''-Dichlorofluorescin diacetate

GW2593-1 1
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2'',7''-Dichlorofluorescin diacetate

GW2593-250 250
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2'',7''-Dichlorofluorescin diacetate

GW2593-50 50
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Melting point SMP20 - EACH

MEL2100 EACH
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AI9100 Melting Point Meter - EACH

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AI9200 Melting Point Meter - EACH

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AgileMelt TM Melting Point Device

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SMP11 Melting Point Apparatus - EACH

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Qual Set Melting Point Range 40C to 240C - 1G

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Melting Point Apparatus Thiele 150mm x 25mm - EACH

7251150X25 EACH
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2,5-Dichlorofluorene

D434445 10mg
EUR 69
Description: 934748-28-4

2,7-Dichlorofluorene

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EUR 132
Description: 7012-16-0

Brass Cooling Plug for Analog Melting Point Apparatus - EACH

MEL2010 EACH
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4,7-Dichloro-fluoren-9-one

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2,7-Dichlorofluorenyl Bromomethyl Ketone

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Melting Point Capilary Tubes 75mm x 1.3-1.4mm Diam Open Both Ends - PK1000

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(E,Z)-9-(4-Chlorophenyl)methylene-5-oxiranyl-2,7-dichlorofluorene

C379640 10mg
EUR 121
Description: 252990-29-7

3,6-Dichlorofluoran

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3,4-Dichlorofluorobenzene 98%

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2,4-Dichlorofluorobenzene

D438810 2.5g
EUR 75
Description: 1435-48-9

Dichlorofluoromethane - 1ML

S-1405 1ML
EUR 95.85

2,4-dichloro-6-fluorophenol

D400715 250mg
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Description: 344-21-8

2,6-Dichloro-3-fluorophenol

D580255 500mg
EUR 3000
Description: 1803854-58-1

3,4-Dichloro-2-fluoroaniline

D434780 100mg
EUR 63
Description: 42965-55-9

Ethyl Dichlorofluoroacetate

E915895 1g
EUR 126
Description: 383-61-9

2,3-Dichloro-5-fluoropyridine

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Description: 185985-40-4

2,6-Dichloro-3-Fluoropyridine

20-abx188187
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3,5-Dichloro-2-fluorobenzoic Acid

D434470 5g
EUR 800
Description: 665022-07-1

4,6-Dichloro-5-fluoropyrimidine

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Description: 213265-83-9

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20-abx182986
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2,4-Dichloro-5-fluoro benzoyl chloride

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11-Dichloro-1-fluoroethane (Freon 141) - 1ML

S-1480 1ML
EUR 68.85

Methyl 3,5-dichloro-2-fluorobenzoate

M295930 5g
EUR 1200
Description: 1522125-32-1

3,5-Dichloro-6-fluoro-2,4-pyridinediamine

D392460 100mg
EUR 1200
Description: 38875-71-7

2,6-Dichloro-4-fluorophenylboronic acid

D475213 1g
EUR 322
Description: 1451392-99-6

3,5-Dichloro-4-fluorobenzotrifluoride

D15335 1G
EUR 343.27

2,4-Dichloro-5-fluoro-6-methylpyrimidine

20-abx184995
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2,4-Dichloro-5-fluoro-7h-pyrrolo[2,3-d]pyrimidine

20-abx181641
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1-(2,6-Dichloro-5-fluoro-4-pyrimidinyl)ethanone

D367060 2.5g
EUR 800
Description: 1289559-65-4

4,6-Dichloro-3-fluoro-1H-pyrazolo[3,4-d]pyrimidine

D434455 100mg
EUR 4500

2-((dichlorofluoromethyl)thio)isoindoline-1,3-dione

D393990 250mg
EUR 800
Description: 719-96-0

4-(1-Bromoethyl)-2,6-dichloro-5-fluoropyrimidine-d3

B590964 100mg
EUR 1800

2,4-Dichloro-6-fluoroquinoline-3-carbonitrile

D580260 1g
EUR 1200
Description: 881313-89-9

1-(3,5-Dichloro-4-fluorophenyl)-2,2,2-trifluoroethanone

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Description: 1-(3,5-Dichloro-4-fluorophenyl)-2,2,2-trifluoroethanone

1-(3,5-Dichloro-4-fluorophenyl)-2,2,2-trifluoroethanone

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Description: 1-(3,5-Dichloro-4-fluorophenyl)-2,2,2-trifluoroethanone

1-(3,5-Dichloro-4-fluorophenyl)-2,2,2-trifluoroethanone

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1-(3,5-Dichloro-4-fluorophenyl)-2,2,2-trifluoroethanone

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Description: 1-(3,5-Dichloro-4-fluorophenyl)-2,2,2-trifluoroethanone

1-(3,5-Dichloro-4-fluorophenyl)-2,2,2-trifluoroethanone

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Description: 1-(3,5-Dichloro-4-fluorophenyl)-2,2,2-trifluoroethanone

4-Amino-3,5-dichloro-6-fluoropyridin-2-ol Potassium Salt

A805775 500mg
EUR 800
Description: 94133-73-0

(R)-5-Bromo-3-(1-(2,6-dichloro-3-fluorophenyl)ethoxy)pyridin-2-amine

B678833 1g
EUR 69
Description: 877399-00-3

4-Bromo-2,6-dichloro-5-fluoro-3-pyridinecarboxylic Acid

B699893 1g
EUR 1109
Description: 1526945-64-1

3-(2,6-Dichloro-5-fluoropyridin-3-yl)-3-oxopropanoic Acid Ethyl Ester

D434200 1g
EUR 58
Description: 96568-04-6

2,4-Dichlorofluobenzene

20-abx184541
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  • 100 g
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3-[(1R)-1-(2,6-Dichloro-3-fluorophenyl)ethoxy]-5-[1-(4-piperidinyl)-1H-pyrazol-4-yl]-2-pyridinamine

20-abx184572
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2-(4,5-Dichloro-2-fluorophenyl)-4-(difluoromethyl)-2,4-dihydro-5-methyl-3H-1,2,4-triazol-3-one

C611000 25mg
EUR 3000
Description: 153833-81-9

4-(5-(3,5-Dichloro-4-fluorophenyl)-5-(trifluoromethyl)-4,5-dihydro-1,2-oxazol-3-yl)-2-methylbenzamide

D422010 250mg
EUR 11200

(R)-tert-Butyl 4-(4-(6-amino-5-(1-(2,6-dichloro-3-fluorophenyl)ethoxy)pyridin-3-yl)-1H-pyrazol-1-yl)piperidine-1-carboxylate

B242310 250mg
EUR 500
Description: 877399-51-4

4-((S)-5-(3,5-Dichloro-4-fluorophenyl)-5-(trifluoromethyl)-4,5-dihydroisoxazol-3-yl)-N-((R)-2-ethyl-3-oxoisoxazolidin-4-yl)-2-methylbenzamide

D422015 250mg
EUR 17000
Description: 1309959-62-3

4-((S)-5-(3,5-Dichloro-4-fluorophenyl)-5-(trifluoromethyl)-4,5-dihydroisoxazol-3-yl)-N-((R)-2-(ethyl-d5)-3-oxoisoxazolidin-4-yl)-2-methylbenzamide

D422019 50mg
EUR 17000

Agarose, Low Melting

RM861-100G 1 unit
EUR 533.55
Description: Agarose, Low Melting

Agarose, Low Melting

RM861-10G 1 unit
EUR 57.22
Description: Agarose, Low Melting

Agarose, Low Melting

RM861-25G 1 unit
EUR 125.79
Description: Agarose, Low Melting

Agarose LM Low Melting

AXY1006 PK10
EUR 3480.06

Agarose, Low melting gel

AB0015 10g
EUR 96.54

HiQTM Low Melting Agarose

A0223-002 25g
EUR 343.2

HiQTM Low Melting Agarose

A0223-010 100g
EUR 699.6

HyAgarose? LM Agarose, Low Melting, 100g

R9012LM-100g each
EUR 452.4

HyAgarose? LM Agarose, Low Melting, 500g

R9012LM-500g each
EUR 1821.6

Agarose LM Low Melting - PK10

AXY1004 PK10
EUR 12779.1

Agarose -LM(melting temperature ≦65℃)

01161-12 25G
EUR 140

Agarose -LM(melting temperature ≦65℃)

01161-54 100G
EUR 357

Agar, powder(melting temperature ~80℃)

01057-05 500G
EUR 95.9

HyAgarose? LM Agarose, Low Melting, 10g

R9012LM-10g each
EUR 120

HyAgarose? LM Agarose, Low Melting, 50g

R9012LM-50g each
EUR 262.8

Tubes Melting Open One End 100mm - PK1000

MEL3004 PK1000
EUR 147.15

HyAgarose™ LM Agarose, Low Melting, 10g

R9012LM 10g
EUR 43.98

AGAROSE, LOW MELT POINT

IB70056 100GM
EUR 377.98

AGAROSE, LOW MELT POINT

IB70057 250GM
EUR 924.25

AGAROSE, LOW MELT POINT

IB70058 500GM
EUR 1768.48

AGAROSE, LOW MELT POINT

IB70059 1KG
EUR 3156.47

AGAROSE, LOW MELT POINT

IB70050 50GM
EUR 254.65

AGAROSE, LOW MELT POINT

IB70051 25GM
EUR 115.63

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-1001 1/pk
EUR 4484.4
Description: Bioscience Mol Bio; Agarose

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 50G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-501 1/pk
EUR 2490
Description: Bioscience Mol Bio; Agarose

Agarose: high melting temperature/medium resolution for all of your standard DNA applications 500 g - EACH

DD4905009 EACH
EUR 1391.85

2,5-Dichlorofuran

D270185 500mg
EUR 800
Description: 42587-83-7

3',4'-Dichloroformanilide

D434401 250mg
EUR 800
Description: 5470-15-5

2,6-Dichloroformanilide

D434400 10g
EUR 215
Description: 10113-35-6

3,5-Dichloro-4-formylbenzoic Acid

L474988 100mg
EUR 64
Description: 153203-80-6

2,5-Dichloro-4'-fluorobenzophenone

D434920 5g
EUR 800
Description: 270903-87-2

Dichloro Fluoro Triazolin-3-one

D439950 50mg
EUR 7600

2,6‐DICHLORO‐5‐FLUORO‐3‐CYANOPYRIDINE

904010 each Ask for price

2,6‐DICHLORO‐5‐FLUORONICOTINIC ACID

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(2E)-4-Bromo-2,4-dichloro-3-formyl-2-butenoic Acid

B679460 25mg
EUR 11200
Description: 441304-66-1

2,6‐DICHLORO‐5‐FLUORONICOTINAMIDE

904011 each Ask for price

2,2-Dichloro-1-(4-fluorophenyl)-2-phenylethanone

D671165 1g
EUR 800
Description: 1403667-07-1

Ethyl 4,6-Dichloro-3-formyl-2-indolecarboxylate

E915910 2.5g
EUR 1200
Description: 153435-96-2

FORCEP, FINE POINT, NO. 3

F335 1EA
EUR 26.24

FORCEPS, FINE POINT, 6.5 IN

F091 1EA
EUR 33.48

FORCEPS, FINE POINT, NO. 10

F340 1EA
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FORCEPS, FINE POINT, NO. 5

F343 1EA
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Tweezers, Slender Tip Fine Point, 115mm

43260005-1 1 Each
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Point Sampler - 1000mm - 316ss - EACH

SAM1057 EACH
EUR 1470.15

Point Sampler - 1500mm - 316ss - EACH

SAM1058 EACH
EUR 1490.4

Point Sampler - 2000mm - 316ss - EACH

SAM1059 EACH
EUR 1502.55

Rat Chromosome X Point Probe

FRWC-20P 20 Tests Ask for price
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Rat Chromosome 1 Point Probe

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IBI ULTRA-FINE POINT SHARPIE

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IBI ULTRA-FINE POINT SHARPIE

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IBI ULTRA-FINE POINT SHARPIE

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luMIX single point stirrer - EACH

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Mouse Chromosome 1 Point Probe

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