Structure of KAP1 tripartite motif identifies molecular interfaces required for retroelement silencing.

Transcription of transposable parts is tightly regulated to stop genome injury. KRAB domain-containing zinc finger proteins (KRAB-ZFPs) and KRAB-associated protein 1 (KAP1/TRIM28) play a key position in regulating retrotransposons. KRAB-ZFPs acknowledge particular retrotransposon sequences and recruit KAP1, inducing the meeting of an epigenetic silencing advanced, with chromatin transforming actions that repress transcription of the focused retrotransposon and adjoining genes.

Our biophysical and structural knowledge present that the tripartite motif (TRIM) of KAP1 kinds antiparallel dimers, which additional assemble into tetramers and higher-order oligomers in a concentration-dependent method. Structure-based mutations within the B-box 1 area forestall higher-order oligomerization with out important loss of retrotransposon silencing exercise, indicating that, in distinction to different TRIM-family proteins, self-assembly just isn’t important for KAP1 operate.

The crystal construction of the KAP1 TRIM dimer identifies the KRAB area binding website within the coiled-coil area close to the dyad. Mutations at this website abolished KRAB binding and transcriptional silencing exercise of KAP1.

Structure of KAP1 tripartite motif identifies molecular interfaces required for retroelement silencing.
Structure of KAP1 tripartite motif identifies molecular interfaces required for retroelement silencing.

This work identifies the interplay interfaces within the KAP1 TRIM accountable for self-association and KRAB binding and establishes their position in retrotransposon silencing.

USP Sol. Dichlorofluorescein TS

USP2305 500ML
EUR 297.6

6-Carboxy-2'',7''-dichlorofluorescein DA

GW0085-100 100
EUR 353.2

6-Carboxy-2'',7''-dichlorofluorescein DA

GW0085-250 250
EUR 668.2

5-Carboxy-2'',7''-dichlorofluorescein DA

GW5240-100 100
EUR 353.2

5-Carboxy-2'',7''-dichlorofluorescein DA

GW5240-250 250
EUR 668.2

CDCF [5-(and-6)-Carboxy-2',7'-dichlorofluorescein] *Mixed isomers*

235-100mg 100 mg
EUR 109
Description: CDCF [5(6)-carboxy-2',7'-dichlorofluorescein] is the fluorescent product of 5(6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate and 5(6)-carboxy-2',7'-dichlorofluorescein diacetate in cells.

CDCFDA [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate] *Mixed isomers*

22025 100 mg
EUR 109

CDCFDA [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate] *Mixed isomers*

22025-100mg 100 mg
EUR 109
Description: It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample.

5-(and-6)-Carboxy-2', 7'-dichlorofluorescein diacetate, succinimidyl ester: (25mg)

90040 25MG
EUR 244.8
Description: Minimum order quantity: 1 unit of 25MG

CDCFDA, SE [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester] *Mixed isomers*

22026 25 mg
EUR 109

CDCFDA, SE [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester] *Mixed isomers*

22026-25mg 25 mg
EUR 109
Description: It is widely recognized that fluorescent labeling of cells is an effective means to determine total cell numbers or how many viable cells exist in a sample.

2'',7''-Dichlorofluorescin diacetate

GW2593-1 1
EUR 388.8

2'',7''-Dichlorofluorescin diacetate

GW2593-250 250
EUR 178.4

2'',7''-Dichlorofluorescin diacetate

GW2593-50 50
EUR 83.3

Melting point SMP20

MEL2100 EACH
EUR 1582.8

Melting Point Tube 75x1.35mm

HAE3151 PK1000
EUR 165.6

AI9100 Melting Point Meter

MEL1000 EACH
EUR 1872

AI9200 Melting Point Meter

MEL1010 EACH
EUR 2224.8

SMP11 Melting Point Apparatus

MEL2000 EACH
EUR 964.8

Melting Point Apparatus SMP30

MEL2102 EACH
EUR 2601.6

SMP10 Melting Point Apparatus

MEL2104 EACH
EUR 1177.2

SMP50 Automatic Melting point

MEL2108 EACH
EUR 7448.4

Melting Point Apparatus SMP30 IQOQ

MEL2102IQOQ EACH
EUR 2864.49

Qual Set Melting Point Range 40C to 240C

AQSMP001 1G
EUR 155.37

Melting Point Apparatus Thiele 150mm x 25mm

7251150X25 EACH
EUR 9.6

Brass Cooling Plug for Analog Melting Point Apparatus

MEL2010 EACH
EUR 42

Melting Point Capilary Tubes 75mm x 1.3-1.4mm Diam Open Both Ends

CAP5010 PK1000
EUR 108

Dichlorofluoromethane

S-1405 1ML
EUR 85.2

2,6-Dichloro-3-Fluoropyridine

20-abx188187
  • EUR 1996.80
  • EUR 727.20
  • 100 g
  • 25 g

3,5-Dichloro-4-fluoropyridine

20-abx180388
  • EUR 510.00
  • EUR 1212.00
  • 1 g
  • 5 g

2,4-Dichloro-5-fluoropyrimidine

20-abx182986
  • EUR 343.20
  • EUR 210.00
  • EUR 243.60
  • 100 g
  • 1 g
  • 25 g

4,6-Dichloro-5-fluoropyrimidine

20-abx185179
  • EUR 444.00
  • EUR 243.60
  • EUR 343.20
  • 10 g
  • 1 g
  • 5 g

11-Dichloro-1-fluoroethane (Freon 141)

S-1480 1ML
EUR 61.2

2,4-Dichloro-5-fluoro benzoyl chloride

20-abx180996
  • EUR 326.40
  • EUR 444.00
  • EUR 276.00
  • 10 g
  • 25 g
  • 5 g

2,4-Dichloro-5-fluoro-7h-pyrrolo[2,3-d]pyrimidine

20-abx181641
  • EUR 2431.20
  • EUR 1095.60
  • EUR 6474.00
  • 1 g
  • 250 mg
  • 5 g

2,4-Dichloro-5-fluoro-6-methylpyrimidine

20-abx184995
  • EUR 1629.60
  • EUR 777.60
  • 1 g
  • 250 mg

2,4-Dichlorofluobenzene

20-abx184541
  • EUR 393.60
  • EUR 276.00
  • 100 g
  • 25 g

3-[(1R)-1-(2,6-Dichloro-3-fluorophenyl)ethoxy]-5-[1-(4-piperidinyl)-1H-pyrazol-4-yl]-2-pyridinamine

20-abx184572
  • EUR 944.40
  • EUR 444.00
  • 25 mg
  • 5 mg

Agarose LM Low Melting

AXY1004 PK10
EUR 11359.2

Agarose LM Low Melting

AXY1006 PK10
EUR 6266.4

Agarose, Low melting gel

AB0015 10g
EUR 96.54

HiQTM Low Melting Agarose

A0223-002 25g
EUR 343.2

HiQTM Low Melting Agarose

A0223-010 100g
EUR 699.6

HyAgarose? LM Agarose, Low Melting, 100g

R9012LM-100g each
EUR 452.4

HyAgarose? LM Agarose, Low Melting, 500g

R9012LM-500g each
EUR 1821.6

Tubes Melting Open One End 100mm

MEL3004 PK1000
EUR 130.8

HyAgarose? LM Agarose, Low Melting, 10g

R9012LM-10g each
EUR 120

HyAgarose? LM Agarose, Low Melting, 50g

R9012LM-50g each
EUR 262.8

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 100G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-1001 1/pk
EUR 4484.4
Description: Bioscience Mol Bio; Agarose

AGAROSE LM, LOW MELTING, MOLECULAR BIOLOGY GRADE, 50G/UNIT, 10 UNITS PER CASE (AL)

AGR-LM-501 1/pk
EUR 2490
Description: Bioscience Mol Bio; Agarose

2,6‐DICHLORO‐5‐FLUORO‐3‐CYANOPYRIDINE

904010 each Ask for price

2,6‐DICHLORO‐5‐FLUORONICOTINIC ACID

904012 each Ask for price

2,6‐DICHLORO‐5‐FLUORONICOTINAMIDE

904011 each Ask for price

Agarose: high melting temperature/medium resolution for all of your standard DNA applications 500 g

DD4905009 EACH
EUR 1237.2

Point Sampler - 1000mm - 316ss

SAM1057 EACH
EUR 1306.8

Point Sampler - 1500mm - 316ss

SAM1058 EACH
EUR 1324.8

Point Sampler - 2000mm - 316ss

SAM1059 EACH
EUR 1335.6

luMIX single point stirrer

MIX1203 EACH
EUR 1329.1

SmartPicks Mini Needle Point

INO0002 PK2000
EUR 210

150x300mm Assembly Point Left

FR01910R EACH
EUR 14.38

420x297mm Fire Assembly Point

FR04549R EACH
EUR 30.12

SmartPicks Large Needle Point

INO0006 PK500
EUR 129.25

420x297mm Assembly Point - Rigid

FR01749R EACH
EUR 30.12

150x300mm Assembly Point Right

FR02010R EACH
EUR 14.38

SmartPicks Standard Semi Point

INO0003 PK1600
EUR 231.6

SmartPicks Standard Needle Point

INO0004 PK1600
EUR 212.4

1-[2-(2,4-Dichlorophenyl)-2-[(2,4-dichlorophenyl)methoxy]ethyl]-1H-imidazole

20-abx182858
  • EUR 427.20
  • EUR 292.80
  • 100 g
  • 25 g

2,3‐DICHLORO PHENOL

904008 each Ask for price

Dichlorophen

D073-25G 25 g
EUR 68.4

Dichlorophen

HY-12638 500mg
EUR 129.6

2,5-Dichloro-3-picoline

20-abx181654
  • EUR 260.40
  • EUR 1128.00
  • EUR 427.20
  • 1 g
  • 25 g
  • 5 g

1,3-Dichloro-2-propanol

20-abx184915
  • EUR 226.80
  • EUR 309.60
  • EUR 661.20
  • 100 g
  • 1 kg
  • 5 kg

3,6-Dichloro-1,2,4,5-tetrazine

20-abx186642
  • EUR 1028.40
  • EUR 2247.60
  • 1 g
  • 5 g

23-Dichloro-1-propene

S-1458 1ML
EUR 85.2

CIS‐1,4‐DICHLORO‐2‐BUTENE

503011 each Ask for price

13-Dichloro-2-propanol

S-1492 1ML
EUR 160.8

3,4-Dichloro-1,2,5-thiadiazole

abx183302-10g 10 g
EUR 276

4,5-Dichloro-3(2H)-pyridazinone

20-abx185173
  • EUR 309.60
  • EUR 243.60
  • 25 g
  • 5 g

3,4-Dichloro-Phe-OH

A6681-100000 100 g
EUR 876
Description: 3,4-Dichloro-Phe-OH

3,4-Dichloro-Phe-OH

A6681-25000 25 g
EUR 466.8
Description: 3,4-Dichloro-Phe-OH

3,4-Dichloro-Phe-OH

A6681-5000 5 g
EUR 262.8
Description: 3,4-Dichloro-Phe-OH

H-3,4-Dichloro-Phe-OH

F-3395.0001 1.0g
EUR 457.2
Description: Sum Formula: C9H9Cl2NO2; CAS# [52794-99-7]

H-3,4-Dichloro-Phe-OH

F-3395.0005 5.0g
EUR 1705.2
Description: Sum Formula: C9H9Cl2NO2; CAS# [52794-99-7]

2,6-Dichloro-1,4-Benzoquinone

abx188186-50g 50 g
EUR 811.2

Hanna Thermometer + 2 Point Ukas

THE4500UKAS2 EACH
EUR 203.67

2,6-dichloropurine

20-abx181665
  • EUR 510.00
  • EUR 1545.60
  • 100 g
  • 500 g

1,2-Dichloroethane

abx186118-01l 0.1l
EUR 243.6

1,2-Dichloroethane

abx186118-1l 1l
EUR 343.2

2,6‐DICHLOROTOLUENE

604011 each Ask for price

2,4‐DICHLOROTOLUENE

504007 each Ask for price

2,6‐DICHLOROANILINE

504008 each Ask for price

3,5‐DICHLOROANILINE

904005 each Ask for price

3,4‐DICHLOROTOLUENE

904013 each Ask for price

Dichloromethane

DC3600 1L
EUR 88.97

Dichloromethane

DC3601 4L
EUR 169.09

Dichloromethane

GK9913-1L 1 l
EUR 60

Dichloromethane

GK9913-2500ML 2500 ml
EUR 84

Dichloromethane

GK9913-500ML 500 ml
EUR 52.8

Dichloromethane

GK9913-1 1
EUR 19.8

Dichloromethane

GK9913-2500 2500
EUR 39.4

Dichloromethane

GK9913-500 500
EUR 14.8

Elga 0.2 Point of Use Filter

WAT4002 EACH
EUR 279.4

replacement tip Kit strong point

INS2094 EACH
EUR 10.35

4,8-Dichloro-1,7-naphthyridine

20-abx182029
  • EUR 2163.60
  • EUR 1144.80
  • EUR 5337.60
  • 1 g
  • 250 mg
  • 5 g

2,6-Dichloro-3-nitrotoluene

20-abx183025
  • EUR 210.00
  • EUR 326.40
  • EUR 243.60
  • 1 g
  • 25 g
  • 5 g

2,6-Dichloro-4-iodopyridine

20-abx183026
  • EUR 1195.20
  • EUR 526.80
  • 100 g
  • 25 g

2,5-Dichloro-3-Iodopyridine

20-abx185004
  • EUR 460.80
  • EUR 760.80
  • 10 g
  • 25 g

2,6-Dichloro-3-Iodopyridine

20-abx185009
  • EUR 326.40
  • EUR 276.00
  • 10 g
  • 5 g

1,2-Dichloro-4-nitrobenzene

20-abx185895
  • EUR 260.40
  • EUR 594.00
  • EUR 226.80
  • EUR 427.20
  • EUR 210.00
  • 100 g
  • 1 kg
  • 25 g
  • 500 g
  • 5 g

2,4-Dichloro-1,8-Naphthyridine

20-abx186183
  • EUR 861.60
  • EUR 2180.40
  • 1 g
  • 5 g

2,4-Dichloro-6-Methylphenol

abx187880-100g 100 g
EUR 560.4

2,6-Dichloro-4-nitroaniline

GK0378-25 25
EUR 23.2

2,6-Dichloroaniline

20-abx183029
  • EUR 260.40
  • EUR 493.20
  • EUR 309.60
  • EUR 393.60
  • 100 g
  • 1 kg
  • 250 g
  • 500 g

3,5-Dichloroaniline

abx188335-1000g 1000 g
EUR 710.4

2,3-Dichloroaniline

20-abx184539
  • EUR 260.40
  • EUR 460.80
  • 25 g
  • 500 g

3,4-Dichloroaniline

20-abx181091
  • EUR 292.80
  • EUR 260.40
  • 1 kg
  • 500 g

2,4‐DICHLORO PYRIMIDINE

504006 each Ask for price

Boc-Tyr(2,6-dichloro-Bzl)-OH

A-1575.0005 5.0g
EUR 135.6
Description: Sum Formula: C21H23Cl2NO5; CAS# [40298-71-3]

Boc-Tyr(2,6-dichloro-Bzl)-OH

A-1575.0025 25.0g
EUR 374.4
Description: Sum Formula: C21H23Cl2NO5; CAS# [40298-71-3]

Boc-Tyr(2,6-dichloro-Bzl)-OH

A-1575.0100 100.0g
EUR 1051.2
Description: Sum Formula: C21H23Cl2NO5; CAS# [40298-71-3]

2,4‐DICHLOROPYRIDINE

904009 each Ask for price

3,4-Dichloro-Phe-OMe.HCl

A6680-1000 1 g
EUR 451.2
Description: 3,4-Dichloro-Phe-OMe.HCl

3,4-Dichloro-Phe-OMe.HCl

A6680-100000 100 g
EUR 27440.4
Description: 3,4-Dichloro-Phe-OMe.HCl

3,4-Dichloro-Phe-OMe.HCl

A6680-25000 25 g
EUR 7882.8
Description: 3,4-Dichloro-Phe-OMe.HCl

3,4-Dichloro-Phe-OMe.HCl

A6680-5000 5 g
EUR 1794
Description: 3,4-Dichloro-Phe-OMe.HCl

3 5 Dichlorophenol 1000mg/L

DPC10001 1L
EUR 584.91

3 5 Dichlorophenol 340mg/L

DPC341 1L
EUR 570.55

Dichloroacetic acid

S-1285 1ML
EUR 45.6

12-Dichlorobutane

S-1359 1ML
EUR 84

11-Dichloroethane

S-1375 1ML
EUR 44.4

12-Dichloroethane

S-1380 1ML
EUR 45.6

11-Dichloroethene

S-1390 1ML
EUR 45.6

23-Dichlorophenol

S-1406 1ML
EUR 93.6

24-Dichlorophenol

S-1410 1ML
EUR 85.2

25-Dichlorophenol

S-1412 1ML
EUR 118.8

26-Dichlorophenol

S-1415 1ML
EUR 45.6

34-Dichlorophenol

S-1416 1ML
EUR 154.8

35-Dichlorophenol

S-1417 1ML
EUR 85.2

2,5-Dichloro-p-xylene

GW3033-100 100
EUR 266.3

2,5-Dichloro-p-xylene

GW3033-500 500
EUR 947.7

2,5-Dichloroaniline

GK8629-50 50
EUR 22.1

22-Dichloro-111-trifluoroethane

S-1476 1ML
EUR 138

12-Dichloro-11-difluoroethane

S-1477 1ML
EUR 222

2,3-dichloro-5-methylaniline

abx182961-1g 1 g
EUR 1395.6

2,4-Dichloro-3-nitropyridine;

abx182984-25g 25 g
EUR 309.6

2,5-Dichloro-3-nitropyridine

20-abx183014
  • EUR 644.40
  • EUR 343.20
  • EUR 243.60
  • 100 g
  • 25 g
  • 5 g

2,6-Dichloro-4-nitropyridine

20-abx183027
  • EUR 260.40
  • EUR 410.40
  • 1 g
  • 5 g

4,5-Dichloro-1,2-Benzenediamine

abx188425-5g 5 g
EUR 243.6

2,6-Dichloro-3-nitropyridine

20-abx184547
  • EUR 276.00
  • EUR 727.20
  • 100 g
  • 1 kg

2,4-Dichloro-5-nitropyridine

20-abx185708
  • EUR 493.20
  • EUR 260.40
  • EUR 360.00
  • 10 g
  • 1 g
  • 5 g

3,5-Dichloro-2-bromopyridine

20-abx185758
  • EUR 260.40
  • EUR 226.80
  • 100 g
  • 5 g

1,2-Dichloro-2-Methylpropane

20-abx186117
  • EUR 260.40
  • EUR 777.60
  • EUR 393.60
  • 1 g
  • 25 g
  • 5 g

2,3-Dichloro-1,4-naphthoquinone

GK4311-5G 5 g
EUR 49.2

14-Dichloro-2-butene (mix of cis and trans)

S-1485 1ML
EUR 45.6

3,6-Dichloro-1,2-benzenedithiol

GT4417-1 1
EUR 102.7

2,3-Dichloro-1,4-naphthoquinone

GK4311-5 5
EUR 12.1

2,5-Dichloropyrazine

20-abx183017
  • EUR 1111.20
  • EUR 360.00
  • EUR 744.00
  • 10 g
  • 1 g
  • 5 g

2,5-Dichlorothiazole

20-abx181003
  • EUR 1429.20
  • EUR 727.20
  • EUR 4352.40
  • 1 g
  • 250 mg
  • 5 g

2,4-Dichloropyridine

20-abx181646
  • EUR 393.60
  • EUR 260.40
  • EUR 210.00
  • 100 g
  • 25 g
  • 5 g

2,5-Dichloropyridine

abx181655-100g 100 g
EUR 376.8

Fmoc-Tyr(2,6-dichloro-Bzl)-OH

B-1280.0001 1.0g
EUR 138
Description: Sum Formula: C31H25Cl2NO5; CAS# [112402-12-7]

Fmoc-Tyr(2,6-dichloro-Bzl)-OH

B-1280.0005 5.0g
EUR 356.4
Description: Sum Formula: C31H25Cl2NO5; CAS# [112402-12-7]

Fmoc-Tyr(2,6-dichloro-Bzl)-OH

B-1280.0025 25.0g
EUR 1298.4
Description: Sum Formula: C31H25Cl2NO5; CAS# [112402-12-7]

Boc-D-Tyr(2,6-dichloro-Bzl)-OH

A-4025.0001 1.0g
EUR 135.6
Description: Sum Formula: C21H23Cl2NO5; CAS# [69541-62-4]

Boc-D-Tyr(2,6-dichloro-Bzl)-OH

A-4025.0005 5.0g
EUR 360
Description: Sum Formula: C21H23Cl2NO5; CAS# [69541-62-4]

4‐AMINO‐2,6‐DICHLOROPHENOL

901009 each Ask for price

2,4‐DICHLORO‐3‐NITRO‐PYRIDINE

904007 each Ask for price

Dichlorophenyl-ABA

HY-113950 1mg
EUR 349.2

23-Dichloroaniline

S-1284 1ML
EUR 85.2

12-Dichlorobenzene

S-1290 1ML
EUR 99.6

13-Dichlorobenzene

S-1295 1ML
EUR 99.6

11-Dichloropropane

S-1429 1ML
EUR 85.2

12-Dichloropropane

S-1430 1ML
EUR 99.6

13-Dichloropropane

S-1435 1ML
EUR 84

22-Dichloropropane

S-1440 1ML
EUR 84

11-Dichloropropene

S-1450 1ML
EUR 158.4

34-Dichlorotoluene

S-1474 1ML
EUR 163.2

34-Dichloroaniline

S-3851 1ML
EUR 99.6

Purite 0.2um Point of Use Filter

SBF02 EACH
EUR 200.4

2,6-Dichloro-9-methyl-9H-purine

20-abx183028
  • EUR 360.00
  • EUR 644.40
  • 1 g
  • 5 g

2,5-Dichloro-4-Methylpyridine

20-abx185005
  • EUR 276.00
  • EUR 811.20
  • EUR 410.40
  • 1 g
  • 25 g
  • 5 g

4,6-Dichloro-1H-pyrrolo[2,3-b]pyridine

20-abx185176
  • EUR 2431.20
  • EUR 594.00
  • EUR 1412.40
  • 10 g
  • 1 g
  • 5 g

4,6-dichloro-1H-pyrrolo[3,2-c]pyridine

20-abx185177
  • EUR 1528.80
  • EUR 4168.80
  • 1 g
  • 5 g

3,6-Dichloro-2-hydroxy benzoic acid

20-abx180033
  • EUR 1228.80
  • EUR 3300.00
  • 1 g
  • 5 g

2,4-Dichloro-5-iodopyrimidine

20-abx180239
  • EUR 1095.60
  • EUR 493.20
  • EUR 292.80
  • 100 g
  • 25 g
  • 5 g

2,6-Dichloro-3-methylpyridine

20-abx180256
  • EUR 343.20
  • EUR 1579.20
  • EUR 661.20
  • 1 g
  • 25 g
  • 5 g

2,4-Dichlorobenzoic acid

20-abx182991
  • EUR 343.20
  • EUR 276.00
  • 1 kg
  • 500 g

2,6-Dichlorobenzoic acid

20-abx183031
  • EUR 260.40
  • EUR 560.40
  • EUR 393.60
  • 100 g
  • 1 kg
  • 500 g

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