Structure of KAP1 tripartite motif identifies molecular interfaces required for retroelement silencing.

Transcription of transposable parts is tightly regulated to stop genome injury. KRAB domain-containing zinc finger proteins (KRAB-ZFPs) and KRAB-associated protein 1 (KAP1/TRIM28) play a key position in regulating retrotransposons. KRAB-ZFPs acknowledge particular retrotransposon sequences and recruit KAP1, inducing the meeting of an epigenetic silencing advanced, with chromatin transforming actions that repress transcription of the focused retrotransposon and adjoining genes.

Our biophysical and structural knowledge present that the tripartite motif (TRIM) of KAP1 kinds antiparallel dimers, which additional assemble into tetramers and higher-order oligomers in a concentration-dependent method. Structure-based mutations within the B-box 1 area forestall higher-order oligomerization with out important loss of retrotransposon silencing exercise, indicating that, in distinction to different TRIM-family proteins, self-assembly just isn’t important for KAP1 operate.

The crystal construction of the KAP1 TRIM dimer identifies the KRAB area binding website within the coiled-coil area close to the dyad. Mutations at this website abolished KRAB binding and transcriptional silencing exercise of KAP1.

Structure of KAP1 tripartite motif identifies molecular interfaces required for retroelement silencing.
Structure of KAP1 tripartite motif identifies molecular interfaces required for retroelement silencing.

This work identifies the interplay interfaces within the KAP1 TRIM accountable for self-association and KRAB binding and establishes their position in retrotransposon silencing.

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2,7-Dichlorofluorescein (DCF) extrapure AR, ACS, 90%

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CDCFDA [5-(and-6)-Carboxy-2',7'-dichlorofluorescein diacetate] *Mixed isomers*

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CDCFDA,-SE-[5-(and-6)-Carboxy-2',7'-dichlorofluorescein-diacetate,-succinimidyl-ester]-*Mixed-isomers*

E37F22026 25-mg
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5-(AND-6)-CARBOXY-2', 7'-DICHLOROFLUORESC

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2',7'-Dichlorofluorescin diacetate

D15325 50MG
EUR 175.32
Description: CAS N° 4091-99-0

2'',7''-Dichlorofluorescin diacetate

GW2593 50mg
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Melting point SMP20 - EACH

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AI9100 Melting Point Meter - EACH

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EUR 2106

AI9200 Melting Point Meter - EACH

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EUR 2502.9

Melting Point Tube 75x1.35mm - PK1000

HAE3151 PK1000
EUR 186.3

AgileMelt TM Melting Point Device

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SMP11 Melting Point Apparatus - EACH

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Melting Point Apparatus SMP30 - EACH

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Qual Set Melting Point Range 40C to 240C - 1G

AQSMP001 1G
EUR 174.79

Melting Point Apparatus Thiele 150mm x 25mm - EACH

7251150X25 EACH
EUR 10.8

2,5-Dichlorofluorene

D434445 10mg
EUR 69
Description: 934748-28-4

2,7-Dichlorofluorene

D434465 1g
EUR 132
Description: 7012-16-0

2,7-Dichlorofluorene

MBS6113380-25g 2.5(g
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2,7-Dichlorofluorene

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2,7-Dichlorofluorene

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Brass Cooling Plug for Analog Melting Point Apparatus - EACH

MEL2010 EACH
EUR 47.25

4,7-Dichloro-fluoren-9-one

D433945 10mg
EUR 282

5-Oxiranyl-2,7-dichlorofluorene

MBS6093845-25mg 25(mg
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5-Oxiranyl-2,7-dichlorofluorene

MBS6093845-5x25mg 5x25mg
EUR 2240

2,7-Dichlorofluorenyl Bromomethyl Ketone

D434480 100mg
EUR 190
Description: 53221-22-0

2,7-Dichlorofluorenyl Bromomethyl Ketone

MBS6040484-50mg 50(mg
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2,7-Dichlorofluorenyl Bromomethyl Ketone

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2,7-Dichlorofluorenyl Bromomethyl Ketone

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Melting Point Capilary Tubes 75mm x 1.3-1.4mm Diam Open Both Ends - PK1000

CAP5010 PK1000
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(E,Z)-9-(4-Chlorophenyl)methylene-5-oxiranyl-2,7-dichlorofluorene

C379640 10mg
EUR 121
Description: 252990-29-7

(E,Z)-9-(4-Chlorophenyl)methylene-5-oxiranyl-2,7-dichlorofluorene

MBS6077808-10mg 10(mg
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(E,Z)-9-(4-Chlorophenyl)methylene-5-oxiranyl-2,7-dichlorofluorene

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(E,Z)-9-(4-Chlorophenyl)methylene-5-oxiranyl-2,7-dichlorofluorene

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3,6-Dichlorofluoran

D15290 25G
EUR 217.25
Description: CAS N° 630-88-6

2,4-Dichlorofluorobenzene

D438810 2.5g
EUR 75
Description: 1435-48-9

3,4-Dichlorofluorobenzene 98%

D15330 20G
EUR 116.16
Description: CAS N° 1435-49-0

2,4-Dichlorofluorobenzene

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Dichlorofluoromethane - 1ML

S-1405 1ML
EUR 95.85

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2,6-Dichloro-3-fluorophenol

D580255 500mg
EUR 3000
Description: 1803854-58-1

2,4-dichloro-6-fluorophenol

D400715 250mg
EUR 800
Description: 344-21-8

2,6-Dichloro-3-fluorophenol

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3,4-Dichloro-2-fluoroaniline

D434780 100mg
EUR 63
Description: 42965-55-9

3,4-Dichloro-2-fluoroaniline

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Ethyl Dichlorofluoroacetate

E915895 1g
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Ethyl Dichlorofluoroacetate

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2,3-Dichloro-5-fluoropyridine

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2,6-Dichloro-3-Fluoropyridine

20-abx188187
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3,5-Dichloro-2-fluorobenzoic Acid

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3,5-Dichloro-2-fluorobenzoic Acid

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4,6-Dichloro-5-fluoropyrimidine

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4,6-Dichloro-5-fluoropyrimidine

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4,6-Dichloro-5-fluoropyrimidine

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2,4-Dichloro-5-fluoro benzoyl chloride

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11-Dichloro-1-fluoroethane (Freon 141) - 1ML

S-1480 1ML
EUR 68.85

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M295930 5g
EUR 1200
Description: 1522125-32-1

Methyl 3,5-dichloro-2-fluorobenzoate

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2,6-Dichloro-4-fluorophenylboronic acid

D475213 1g
EUR 322
Description: 1451392-99-6

3,5-Dichloro-6-fluoro-2,4-pyridinediamine

D392460 100mg
EUR 1200
Description: 38875-71-7

2,6-Dichloro-4-fluorophenylboronic acid

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2,6-Dichloro-4-fluorophenylboronic acid

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3,5-Dichloro-4-fluorobenzotrifluoride

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2,4-Dichloro-5-fluoro-6-methylpyrimidine

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2,4-Dichloro-5-fluoro-7h-pyrrolo[2,3-d]pyrimidine

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4,6-Dichloro-3-fluoro-1H-pyrazolo[3,4-d]pyrimidine

D434455 100mg
EUR 4500

1-(2,6-Dichloro-5-fluoro-4-pyrimidinyl)ethanone

D367060 2.5g
EUR 800
Description: 1289559-65-4

4,6-Dichloro-3-fluoro-1H-pyrazolo[3,4-d]pyrimidine

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1-(2,6-Dichloro-5-fluoro-4-pyrimidinyl)ethanone

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2-((dichlorofluoromethyl)thio)isoindoline-1,3-dione

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Description: 719-96-0

2-((dichlorofluoromethyl)thio)isoindoline-1,3-dione

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4-(1-Bromoethyl)-2,6-dichloro-5-fluoropyrimidine-d3

B590964 100mg
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4-(1-Bromoethyl)-2,6-dichloro-5-fluoropyrimidine-d3

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2,4-Dichloro-6-fluoroquinoline-3-carbonitrile

D580260 1g
EUR 1200
Description: 881313-89-9

1-(3,5-Dichloro-4-fluorophenyl)-2,2,2-trifluoroethanone

MBS5786531-10mg 10mg
EUR 160

1-(3,5-Dichloro-4-fluorophenyl)-2,2,2-trifluoroethanone

MBS5786531-25mg 25mg
EUR 200

1-(3,5-Dichloro-4-fluorophenyl)-2,2,2-trifluoroethanone

MBS5786531-50mg 50(mg
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1-(3,5-Dichloro-4-fluorophenyl)-2,2,2-trifluoroethanone

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1-(3,5-Dichloro-4-fluorophenyl)-2,2,2-trifluoroethanone

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4-Amino-3,5-dichloro-6-fluoropyridin-2-ol Potassium Salt

A805775 500mg
EUR 800
Description: 94133-73-0

4-Amino-3,5-dichloro-6-fluoropyridin-2-ol Potassium Salt

TRC-A805775-500MG 500 mg Ask for price

2,6-Dichloro-1-fluoropyridinium tetrafluoroborate

sc-238397 100 mg Ask for price

2,6-Dichloro-1-fluoropyridinium tetrafluoroborate

sc-238397A 1 g Ask for price

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